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For the "90 k Reference" pool, all of the shRNA plasmids were combined at approximately equal concentrations, while a dilution series pool, the "90 k Dilution", contained four sub-sets of ~3,500 shRNAs each that were diluted 2-fold, 4-fold, 10-fold or 20-fold with respect to their counterparts in the 90 k Reference pool.
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For countries with short series, pooling was necessary.
A pooled analysis by Carlson and Roth [12] found the proportion of patients with low-risk recurrence scores receiving chemotherapy to range between 0 and 16% across fourteen series (pooled mean 5.8%).
The sides will clash at Thomond Park at 1345 BST after Munster qualified as top seeds and Ulster as eighth seeds after the final series of pool games.
The sides play a series of pool matches before finals to decide who gets the three places in the higher division next season.
A series of pool fire tests was conducted using a flat, unconfined model ceiling with dimensions of 2.5 m (D ×3.0 m (L) and changing the ceiling inclination angle of up to 40°.
For instance, imagine that you saw two random people named Hurt and Spencer playing a series of pool games at a bar.
When a series of pool floaters happen, we have to open our books and balance the facts and ask ourselves the simple three-part question: Is this the work of one person?
The experimental design for this phase of array testing must meet the following three criteria: 1) Require a minimal number of arrays and pools; 2) Introduce transcripts as series of pools with balanced cRNA load that are made at a central site, rather than in individual testing labs; and 3) Use the same pools on both one-color and two-color platforms.
These low-key places in Bali make every day an indulgence: Air Panas Banjar has hot springs percolating down through a series of pools amid lush tropical plants; soak in the top pools, or get a pummelling massage from the water as it falls into the lower pools.
To ensure efficiency in all species and tissues, a standard curve was generated for each species and tissue (n = 6 dilution series) using pooled equimolar amounts from three samples from each condition, diluting the pool 10-fold and then producing a 5-fold dilution series (six points).
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