Sentence examples for serial virus from inspiring English sources

The engineered receptor tropisms were stably maintained during multiple serial virus passage without reversion to native receptor usage, even on cells offering the choice of both native and alternative receptors.

Using a combination of targeted mutagenesis followed by serial virus passage in ferrets, we investigated whether A/H5N1 virus can acquire mutations that would increase the risk of mammalian transmission (34).

To examine effect of RBV on virus production, SFM containing infectious particles was collected 24 or 48 h p.i., and viral titrations were performed in 96-well plate format by infecting BHK21 (for VSV) or Vero cells (for SeV) with serial virus dilutions.

Numbers shown on the table represent the serial virus dilution.

Virus titers were determined by inoculation of 10-fold serial virus dilutions into CEF cells and counting by GFP autofluorescence under fluorescence microscopy as described previously (Engel-Herbert et al. 2003; Zhang et al. 2005).

After 24 h, the media were replaced with serial diluted virus solution, and the cells were cultivated under the conditions of 5% CO2 and 37°C.

The EID50 titers of 6 viruses were determined by serial titration of viruses in 10-day-old embryonated SPF chicken eggs at 37°C.

Monolayer cultures of Vero cells (for KOS and hrR3) or E5 cells (for ICP4 mutant viruses) grown in six-well dishes were infected with serial dilutions of virus.

To estimate the Oral Infectious Dose 50% values (OID50), frozen stocks of viruses were thawed at 37°C and four to five 10-fold serial dilutions of virus were made in L-15 medium followed by mixing the samples with an equal volume of defibrinated sheep blood.

Serial dilutions of virus supernatant, from 0.5 log to 7 log, were performed before adding the virus dilutions onto the plates in quadruplicate.

The sensitivity was determined by testing serial dilutions of virus and RNA standard.