All these modulation types are used in serial transfer mode.
However, the cumbersome process required many serial transfers to obtain these isolates.
Mutants were selected by serial transfers in AM1 mineral salts medium with 10% xylose.
A serial transfer of 50 mg of cells (dry weight) into fresh medium was performed every 24 h.
Numerical models are applied to predict productivity and explicitly show the equivalency of alternative configurations to buffered serial transfer lines.
Ethanol production varied between clones immediately after transformation, but tended to converge to a single value after several rounds of serial transfer.
These evolutionary methods can consist of serial transfers or engineered methods, such as global transcription machinery engineering and the SCALEs expression libraries.
Conditions in competitions were equivalent to those during serial transfer.
Similar calculations were applied to interpret the outcome of serial transfer experiments by Zhao and coworkers [13].
For serial transfer experiments, cultures were grown overnight and total cell numbers were determined microscopically using a counting chamber (ZINTL, Western Germany).
