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The trephine burs including the bone biopsies were fixed in 4% formalin for 5 7 days, rinsed in water, and dehydrated in serial steps of ethanol (70, 80, 90, and 100%), remaining for 1 day in each concentration.
CFGE combined with Cre/lox recombination is put forward as powerful approach for targeted genome editing, involving serial steps of manipulation to expedite the genetic accessibility of S. meliloti as chassis.
After addition of MgCl2 to a concentration of 5 mM, a concentration kept through-out the purification process, the dialysed extract was centrifuged once more, and this final extract was subjected to serial steps of chromatography with adequate dialyses between each step (all steps performed at 12°C).
Recent findings reveal that lncRNAs are implicated in serial steps of cancer development [ 113].
In the serial steps of evaluation, different groups of SSc patients and control subjects were used, therefore a selection bias in our study cannot be completely excluded.
The initial 47S transcript undergoes serial steps of rRNA processing to generate mature 18S, 5.8S, and 28S rRNAs, starting with excision of the Leader sequence from the 5′ external transcribed spacer (ETS) at about +650 nucleotide in mice and at the +414 site in humans.
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Conversely, unconscious information fails to be sequentially dispatched to the successive steps of a serial task and, therefore, remains blocked within a fixed representational state, where it slowly decays.
The practice of e-MBE requires four serial steps: 1. Identification of the problem and precise formulation of an answerable question about the problem.
"Our conscious minds tend to think in terms of serial steps," says Steve Scott, chief technology officer at Cray, a storied maker of supercomputers.
This genetically programmed process includes serial steps ranging from initiation of the phagophore assembly site to the formation of double membrane vesicle complex termed autophagosome, which encapsulates cytoplasmic organelles and proteins and subsequently fuses with lysosomes for the degradation of these intracellular constituents [ 85].
The BLATCAT program can significantly reduce serial steps in comparing specific regions of six representative primate genome sequences and support both position and sequence based approach.
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