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"Our conscious minds tend to think in terms of serial steps," says Steve Scott, chief technology officer at Cray, a storied maker of supercomputers.
The practice of e-MBE requires four serial steps: 1. Identification of the problem and precise formulation of an answerable question about the problem.
CFGE combined with Cre/lox recombination is put forward as powerful approach for targeted genome editing, involving serial steps of manipulation to expedite the genetic accessibility of S. meliloti as chassis.
Tegobuvir could not be seen to interact with NS5B at all while filibuvir interacted in a single reversible step (except at low temperatures) and VX-222 in two serial steps, interpreted as an induced fit mechanism.
This technique also allows the efficient parallelization of the algorithm and leads to higher parallel speedups than do other boundary condition implementations, many of which involve inherently serial steps; this is important, because the expense of meshfree computations makes parallelization crucial for large-size problems.
Each suspension was diluted in four tenfold serial steps in diluted Kligman buffer (0.05% Triton X-100 in 0.0375 M phosphate buffer, pH 7.9).
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The final diagnosis had always been done by a meticulous examination of serial step sections.
Clustering requires an additional serial step, while the identity-by-descent report includes a pairwise population concordance test which does not benefit from bit-level parallelism, but speedups for both remain greater than 100x on 64-bit systems.
This is due to the fact that while alignment itself can be parallelized, all alignments for a given chromosome must be completed before joint variant-calling can be performed, effectively making the final step a single serial step resulting in each NGS sample completed and ready at the total wall time of the entire workflow.
Although this study was hypothesis driven, we emphasize that verification requires future studies in which the entire right and left temporal lobes of forensic infant autopsied cases are examined in serial step sections with precise matching of anterior to posterior levels, and in depth immunocytochemical studies to characterize cell markers of interest.
In all cases, serial-step 5 μm sections were cut from paraffin-embedded tissue samples and stained with hematoxylin-eosin for histological examination.
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