We next screened a serial of site-directed mutants in Loop 1 and Loop 2 and measured their catalytic activities towards UMP and dUMP (Figs. 6A, 6B and S5A).
Successful recombinants contained serial loxP sites with one containing the lox66 and lox71 inverted repeat mutants and another with the wild-type inverted repeats.
An MRI substudy comprising 98 patients (placebo n=23, 50 mg n=37, 100 mg n=38) at eligible MRI substudy sites had serial spine MRI scans (sagittal plane, 1.5T scanners, T1 and short tau inversion recovery sequences) at baseline and weeks 14 and 104.
Double mutations where two of the three histidines were both mutated to alanine were created by conducting serial rounds of site directed mutagenesis as follows: H252A/H232A, H252A/H306A, H232A/H306A.
A serial amino acid sites are identified as co-evolution positions while significant positive selection signals are detected in the single copy psbA gene from the fern species.
Although different scanner models were used across the 4 study sites, serial scanning was always performed on the same scanner for each patient.
The Clapham North shelter was a site of serial illicit trespass.
You also enter in your serial code, which the site requires photographic evidence of to verify.
Founded by serial entrepreneurs Tim Stracke and Dirk Schwartz, the site has been live since 2003 but has just recently streamlined its operations to offer a sort of watch search engine that allows users to find timepieces of note from almost anywhere in the world.
It was the execution site of serial killer John Wayne Gacy and the home of the last functioning panopticon, or "roundhouse" unit, in the country.
All specimens collected every day were recorded in the first register book (which included the date and time of specimen collection, serial number, initials of site of collection and pick up personnel) using assigned serial numbers.
