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Serial section electron tomography was completed as described using semi-thick (300 nm) serial sections were collected on formvar-coated copper slot grids.
Serial section scanning electron microscopy.
Serial section transmission electron microscopy.
Repeated EBSD measurements on each serial section show that these grains were actually identical to the abnormally growing grain, being connected three dimensionally.
Serial section technique for 3-D visualisation was employed to investigate the crystallographic grain orientation correlation with fracture mechanics during HCF loading.
SIA allows ion ablation to remove nanometer sections of tissue for SEM imaging, resulting in serial section data collection for three-dimensional reconstruction.
On the same slide, a serial section was treated without the primary antibody as a control for secondary antibody specificity.
The data reported here are from 7 um serial section series undertaken for each of the diseases listed.
A serial section is also treated without any primary antibody, as a control for the secondary antibodies specificity.
If vessels were found that had CD31 stain but lacked another marker in a serial section, the biopsy was scored as negative for that marker.
Staining was not observed in the lymphocyte-rich area of the granuloma or when we stained a serial section with the normal rabbit IgG control antibody (Figure 1B).
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