Sentence examples for serial repopulation from inspiring English sources
Primary recipient mice were euthanized 6 months after transplantation, BM were isolated from the tibiae and femur, and 2 × 10 BM cells were injected into secondary recipients to test serial repopulation potential.
Likewise, SIRT1-deficient bone marrow cells confer stable bone marrow reconstitution in competitive repopulation and serial transplantation experiments [ 50].
In contrast, HSCs in young Smurf2-deficient mice also outperformed WT HSCs in serial transplantation and competitive repopulation.
Supporting this notion, we found that Smurf2-deficient HSCs of aged mice substantially outperformed aged WT HSCs in competitive repopulation and serial transplantation.
Indeed, using the serial transplantation assay to measure repopulation potential and replication life span, experiments have demonstrated there is no difference in these properties between stem cells taken from old multiparous and nulliparous mice [ 13].
Surprisingly, Tet2-mutated HSCs showed enhanced self-renewal and long-term repopulation (LTR) capacities in serial transplantation assays as compared to WT. Tet2-mutated HSCs are extremely competitive over WT counterparts in vivo, as peripheral engraftment of the former became increasingly dominant when cells were transplanted serially into the secondary and tertiary recipients.
STAT5 was much more critical for early hematopoiesis including HSC self-renewal[10], whereas Gab2 was active in multilineage hematopoietic repopulation but was not essential for serial transplantation ability[26].
Complete liver repopulation was obtained from FAH+FoxM1+ double-positive hepatocytes after two rounds of serial transplantation (data not shown).
After FoxM1-SB plasmid injection, livers of primary recipients with high level of repopulation (>50% FAH+FoxM1+ hepatocyte on sections) were perfused to isolate hepatocytes and perform serial transplantation.
Kreso et al., followed the repopulation dynamics of 150 single lentivirus-marked lineages from ten human CRCs through serial xenograft passages in mice using DNA copy number profiling, sequencing and lentiviral lineage tracking.
