The proposed MM has four degrees-of-freedom (dof) serial manipulator mounted on three of mobile bases.
For immunohistochemistry, all samples were prepared as 5-μm-thick serial sections mounted on glass slides.
Four-micromolar thick serial sections, mounted on poly- L-lysine-coated glass slides, were dewaxed, rehydrated through multiple graded ethanol solutions, treated with 3% hydrogen peroxide for 5 min to inactivate endogenous peroxidases, and washed in distilled water.
Serial sections were mounted on microscope slides, dehydrated and mounted with coverslips with Permount (Sigma), and visualized and imaged with a conventional stereomicroscope equipped with a digital camera.
Serial sections were mounted on formvar-coated, single-slot copper grids, stained with lead citrate at room temperature for 5 min, and washed with distilled water.
We mounted serial sections on slides, removed the paraffin, and rehydrated the sections.
Formalin-fixed paraffin-embedded tissue was cut in serial sections and mounted on glass slides.
4-µm-thick serial sections were mounted on glass slides and stained with hematoxylin and eosin.
Paraffin serial sections were mounted on Superfrost Plus microscope slides (Fisher Scientific, Fair Lawn, NJ).
If negative, six exact serial sections are mounted, and separately numbered 1 6.
Serial sections were mounted onto glass slides and stained with haematoxylin and eosin and Safranin-O.
