Exact(1)
TLR ligand concentrations ranging from (LPS: 10-0.31 pMcomoland(Pam3CSK4Pam3CSK4: 10-0.31 µg/ml) and non-TLR ligands (mouse IP-10 and TNFα: 1000-31 pg/ml) were made in duplicate wells using sterile PBS by serial fold dilutions in the 96-well tissue culture plates at 50 µl final volumes.
Similar(59)
Serial-fold dilutions of sera in PBS and 0.02%Tween 20 were loaded into duplicate wells.
Standard curves for the viral gene were generated by using a plasmid with serial 10 fold dilutions.
MIC and MFC were investigated by serial two fold microbroth dilution method.
Two or more consecutive serial two fold dilutions exhibiting a %bias less than 20% identify a range of linearity.
Each stock solution was diluted to prepare serial two-fold dilutions at concentrations in the range of 500 3.125 μg/mL.
Serial 10-fold dilutions of the suspensions were prepared, and 100 µl aliquots were streaked on Luria Bertani (LB) plates.
with serial 10-fold dilution of virus.
Positive samples were subsequently titrated using serial 2-fold dilutions.
Standards were prepared as serial 3-fold dilutions in quadruplicates.
Numbers of viable bacteria were estimated by limiting dilution using serial ten-fold dilutions of samples.
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