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The serial flow perfusion through the bioreactor increased cell-fluid contact area for effective mass exchange.
The aim of this case study was to (1) investigate differences in mechanical exposure (i.e. mechanical forces arising in the body of the operator) between two production strategies: long-cycle parallelised flow assembly (OLD) and conventional serial flow assembly (NEW), and (2) estimate potential changes in job exposure as a consequence of waste reduction when rationalising a line system.
Cellular phenotype and viability of FACS purified cells was confirmed by serial flow cytometric analysis prior to injection for tumorigenicity studies.
Erythrocyte lifespan was determined by in vivo biotinylation of erythrocytes and serial flow cytometric detection of biotinylated red cells using streptavidin-PE, as described [23].
In the current study, we monitored immune response to therapy by serial flow cytometric evaluation of PBMC populations (T-cell subsets, NK cells, and T-reg cells), serum-soluble IL2 receptor determination, and PBMC response to tetanus toxoid.
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These included: a trial of inhaled β2-agonist during exercise, serial peak flow monitoring, peak flow with reversibility testing and in one case full blood count, chest radiograph, electrocardiogram and echocardiogram.
Here, a serial reacting flow solver was used as a starting point.
Finally, a mode decision module uses serial data flow to find the best mode by comparing the total minimum Lagrangian costs.
The program simulates the conditions in a system of multiple tanks that may be arranged in sets of parallel raceways with serial water flow.
Serial blood flow measurements were performed sequentially in the ischemic (left) and non-ischemic (right) hind limbs and were analyzed by a LDBF image analyser and expressed as pseudocolour images (Figure 1A).
a Methods for isolating single cells from abundant cell populations include: robotic or manual micromanipulation, serial dilution, flow-sorting, microfluidic methods, and laser-capture microdissection; b methods for isolating single cells from rare cell populations include: CellSearch™, DEP-Array™, CellCelector™, MagSweeper™, and nanofilters [16].
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