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Similar to serial extraction, the methanol solvent extracted the highest level of phenolics).
We hypotonically extracted soluble proteins from purified schizonts or merozoites, followed by serial extraction of the insoluble pellet with a high-salt buffer and sodium carbonate buffer, pH 11.0 to separate peripheral from integral membrane proteins.
Using a serial extraction method, we also investigated the levels of triton-soluble and formic acid-soluble Aβ.
This analysis is one of the important criteria in determining whether a treatment plan should involve serial extraction, guidance of eruption, space maintenance, space regaining, or merely periodic observation [3, 4].
On the other hand, performing interceptive orthodontic procedures (such as serial extraction) at the right time is very important.
Using serial extraction with hexane, CH2Cl2, and MeOH, Motta et al. [ 20] identified a great diversity of diterpenes, especially in the CH2Cl2 extract.
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Lanes 1 5: serial extractions from polished seed; lanes 6 7: serial extractions from polishing waste.
Between the serial extractions, the funnels were vigorously shaken for 2 min.
Figure 6 shows the results obtained in 5 and 2 serial extractions carried out on the polished seed flour and the polishing waste, respectively.
In serial extractions, there was a shift of dysferlin from soluble phase in high salt/Triton X-100 to the more insoluble SDS fraction in AD.
The dry pellet was reconstituted in 300 μL of water, and lipids were removed by serial liquid liquid extraction with 500 and 300 μL of chloroform, respectively.
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