But if this Court holds that it does, how will the government explain registrations like MARIJUANA FOR SALE, CAPITALISM SUCKS DONKEY BALLS, LICENSED SERIAL KILLER, YID DISH, DIRTY WHOOORE CLOTHING COMPANY, and MURDER 4 HIRE?[4].
Cloning was performed in a 96 well cell culture dish by serial dilution starting with 60-120,000 cells.
In preparation for DNA extraction, diapausing eggs were washed three times in distilled water, by way of serial transfer between Petri dishes, placed in individual sterile 200 μL centrifuge tubes containing 35 μL of InstaGene Matrix [Bio-Rad Laboratories] and had their contents released by crushing them against the side of the tube.
A total of 2 × 10 cells were then added to a 96-well mictrotiter dish, followed by serial 5-fold dilutions and pronging onto selective plates.
cos cells were plated in 96 well dishes and incubated, in serial dilution, with either BAAV or rAAV4 vector encoding a CMV-eGFP expression cassette in either the presence or absence of 20mg/ml chitotriose (Sigma).
Aliquots of serial dilutions were placed on agar dishes (Difco Laboratories) and incubated at 37 °C.
Cells were plated by serial dilution into 60-mm culture dishes, allowed to adhere overnight and then treated with different doses of IR.
Monolayer cultures of Vero cells (for KOS and hrR3) or E5 cells (for ICP4 mutant viruses) grown in six-well dishes were infected with serial dilutions of virus.
Samples were removed as a function of time up to 5 h later, centrifuged, washed and plated in serial dilution in plastic tissue culture dishes containing 5 ml medium.
Briefly, approximately 2 × 105 TE671 cells were plated in six-well culture dishes and infected with serial 10-fold dilutions of viral vectors (10-1 to 10-5) in serum free medium.
