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Material defects may cause serial defects which can result in a large number of failures concerning one product.
In order to reveal a possible respiratory growth defect, serial dilutions of the strains were incubated on minimum medium supplemented with either dextrose or glycerol, at 28°C and 37°C.
After serial debridements, a soft tissue defect that constituted 70% of tibial surface was produced (Fig. 1a, b).
Serial sections were generated through each defect; approximately 100 slides were made through each defect.
Some Mohs surgical excisions and other serial excisions leave partial-thickness defects.
Finally, the CPC or P24/CPC microspheres were implanted into rabbit femoral condyle defects and P24/CPC serial groups were observed for 4, 8, and 12 weeks.
Microscopic examinations of the conidia and mycelia morphology of all 4 serial strains revealed no obvious defects or changes.
For some colonic defects >2.7 cm, serial OTSC placement can be used with success, but closure may be associated with luminal obstruction depending on the location and configuration of the initial resection.
This increase in pregnancy losses and perinatal deaths could be caused by gene expression defects accumulated throughout the serial cloning procedures, which could be detected in blastocysts, although no phenotypic alterations are observed at this stage.
The samples were cut into 10 μm serial sections from the center of the defect using a microtome (Reichert Jung polycot).
These results are consistent with the temperature-sensitive respiratory growth defect observed in the mzm1Δ/mzm1 D25N serial dilution spot test.
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Justyna Jupowicz-Kozak
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