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A total of 226 duck sera samples were tested using the assay.
In analysing the blood samples of the immunocompromised patients 5.4% (3/56) EDTA blood samples and 16.1% (9/56) sera samples were tested fuPCR-positive, whereas all samples of 30 healthy individuals with no signs of immunological deficits were tested negative by fuPCR.
All 46 sera samples were tested for seroreactivity with the three proteins (Table 2).
The various sera samples were tested at a 1 100 dilution.
All 171 sera samples were tested for anti-Pkn1, anti-OmpA, and anti-LigA antibodies (Table 3).
Data summarized in Figure 2. In all, one hundred twenty six sera samples were tested for antimicrobial activity (Table 1).
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We obtained convalescent sera 19 21 days after the acute sera; these samples were tested in parallel with the acute sera.
Twenty-two soldiers had predeployment and postdeployment sera available; samples were tested for phase I and phase II antibody to Q fever by using IFA.
Sera from cryopreserved samples were tested in duplicate using microtiter plates (NUNC) coated with antigen.
368 sera samples and 6 manure samples were tested in this study.
The OD of each well was measured at 450 nm, and results were calculated as follows: The Final A450nm = [A450 nm of test sera on West Nile antigen] – [A450nm of normal sera on West Nile antigen] Serum samples were tested for virus-neutralizing (Nt) antibodies to WNV (NY99-4132 strain, 1 Vero passage) by the plaque-reduction neutralization test in Vero cell culture.
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