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The collected sera samples were stored at −70 °C before being used in the study.
Sera samples were stored at −20 °C until used.
All sera samples were stored at -80°C until used.
The sera samples were stored frozen at −20°C until testing in the HAI assay.
Blood samples were collected at d0 and at every 7 days by venipuncture using tubes without anticoagulant, and sera samples were stored at −20°C until analysis of inflammatory cytokines.
For instance, since the sera samples were stored at -20° C, we could not measure n-3 or n-6 fatty acids, also found in sea food, and which are beneficial to pregnancy outcomes.
Similar(53)
Sera and plasma samples were stored at −80°C until their use.
All sera and CSF samples were stored in aliquots at −80°C until use.
Sera from enrollment blood samples were stored at –35°C until shipment on dry ice to the CDC (Atlanta, GA, USA) for organochlorine analysis.
Sera prepared from the blood samples were stored at −80°C until analysed by ELISA.
After 1 h, blood samples were centrifuged at 700 g for 15 min, and sera samples were collected and stored at −30 °C until analysis within 48 h.
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