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The five tumor library lysate pools displaying the most significant increases in reactivity between normal and tumor sera samples were selected (p-values ranged from 2.04E-5 2.04E-5E-3; fdr q-values ranged from 3.78E-3 to 1.86E-2).
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Melanoma serum samples were selected from a frozen collection of sera banked at Heidelberg University, Germany in the period from April 2002 to November 2004.
A random subset of 373 serum samples was selected from the 612 EIA-negative sera and tested using LFA: 371 of 373 (99.5%) were LFA negative, and 2 (0.5%) were LFA positive.
For each of these validation pools, the positive and negative samples were selected using simple random sampling from the archived sera.
The sample was selected intentionally.
The collected sera samples were stored at −70 °C before being used in the study.
A total of 226 duck sera samples were tested using the assay.
Moreover, eight human sera samples were analyzed with recoveries of 85.0 107.5%.
The clotted blood samples were centrifuged at 3000 rpm for 10 min and the clear sera samples were aspirated off and stored frozen at − 80 °C for biochemical analyses.
The clotted blood samples were centrifuged at 3000 rpm for 10 min, and the clear sera samples were aspirated off and stored frozen at −80 °C for immunobiochemical analyses.
Rabbits were bled 2 weeks after the second dose and sera samples were collected.
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