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In general, the levels in OA sera samples were only slightly higher than the normal levels and those in the rheumatoid arthritis sera were markedly higher.
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The collected sera samples were stored at −70 °C before being used in the study.
A total of 226 duck sera samples were tested using the assay.
Moreover, eight human sera samples were analyzed with recoveries of 85.0 107.5%.
The clotted blood samples were centrifuged at 3000 rpm for 10 min and the clear sera samples were aspirated off and stored frozen at − 80 °C for biochemical analyses.
The clotted blood samples were centrifuged at 3000 rpm for 10 min, and the clear sera samples were aspirated off and stored frozen at −80 °C for immunobiochemical analyses.
Rabbits were bled 2 weeks after the second dose and sera samples were collected.
All distilled sera samples were analysed for D2O concentration in triplicate and the values averaged.
Sera samples were diluted with saline.
Sera samples were prepared according to manufacturer's instructions.
Sera samples were obtained from these patients before treatment.
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