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Sera samples were made into aliquots and stored at -20°C for short term storage (<6 months) and -80°C for long term storage (>6 months).
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Briefly, DTS samples were made in the laboratory by drying aliquots of known positive and negative sera in small tubes and panels were sent to the clinics.
The clotted blood samples were centrifuged at 3000 rpm for 10 min and the clear sera samples were aspirated off and stored frozen at − 80 °C for biochemical analyses.
The clotted blood samples were centrifuged at 3000 rpm for 10 min, and the clear sera samples were aspirated off and stored frozen at −80 °C for immunobiochemical analyses.
Sheep sera samples were routinely examined during the immunization process, and the selection of material for library construction made based on the best measured responses.
The collected sera samples were stored at −70 °C before being used in the study.
A total of 226 duck sera samples were tested using the assay.
Moreover, eight human sera samples were analyzed with recoveries of 85.0 107.5%.
Rabbits were bled 2 weeks after the second dose and sera samples were collected.
All distilled sera samples were analysed for D2O concentration in triplicate and the values averaged.
Sera samples were diluted with saline.
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