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Sera samples were kept frozen at -40°C until analysis in the microbiology laboratory of the Hospital Clinic of Barcelona.
Samples of blood from treated animals were obtained after the second and third administrations and sera samples were kept frozen until use for immunochemical experiments.
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Adequate amounts of the samples were kept to analyze for glucose, total protein, LDH, cholesterol (CHOL) and triglycerides (TGs) in pleural effusions and sera.
Samples were kept on ice until analysis.
Samples were kept frozen until use.
All samples were kept on ice.
The blood samples were kept at room temperature for 30 min and then centrifuged at 1,000× g at 4°C for 20 min to obtain sera.
Samples were kept on ice.
Samples were kept at -80°C until analysis.
The samples were kept cool during shipment.
Unfixed samples were kept frozen at −80°C.
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