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Sera samples were diluted 1∶200, then serially diluted three-fold in PBS-T with 1% goat serum, and incubated overnight at 4°C.
Sera samples were diluted with saline.
Based on our preliminary studies (data not shown), thawed human sera samples were diluted 1/100 with TBS and 100 μl of each diluted human sera were then added to the 96-well plate in duplicate.
After washing 5 times with PBS 0.05% Tween 20 (PA, Merck), sera samples were diluted to 1/100 in PBS and were added in duplicate to the wells and incubated for 2 hours.
To demonstrate that the AuNP-based immunoassay can accurately quantify target molecules in biological samples, the patient sera samples were diluted using fetal bovine serum by 10 000-fold to correspond to the linear range of the calibration curve.
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The negative and positive control sera from cattle supplied with the kit were used and test serum samples were diluted 1 2.
The serum samples were diluted 1∶100.
All the serum samples were diluted 1∶50.
Briefly, calibrators, control sera and patients' sera (stored samples) were diluted and incubated with a mouse monoclonal antibody against PGRN adsorbed onto the microtiter plate wells.
In brief, for PGRN, calibrators, control sera, and patients' sera (stored samples) were diluted and incubated with a mouse monoclonal antibody against PGRN, adsorbed onto the microtiter plate wells.
All samples were diluted 1 100.
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