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Anti-AVA sera pool AVR801 was used as a positive control and standard curve.
The mean OD obtained using negative sera pool plus three times standard deviation was used as the cut-off value.
A sera pool from 12 healthy individuals was used as a control.
All 88 peptides of each dataset were screened using a tumor sera pool (composed of 20 sera) obtained from ovarian cancer patients and a reference sera pool (composed of 10 sera) from non-cancer female patients.
Figure 2 gives the raw ELISA signal intensity distributions for all peptides tested using the tumor sera pool (20 samples) and the reference sera pool (10 samples), and compares candidate epitopes selected from upregulated (UP) and downregulated (DOWN) genes.
The same process was repeated using a sera pool from tuberculosis patients and M. tuberculosis protein in order to obtain anti- M. tuberculosis antibodies.
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To further support our observations of a significant seroprevalence of anti-Pkn1 antibodies in chlamydial infections, the purified chlamydial proteins were immunoblotted using pooled sera samples as shown in Figure 5. Sera pooled from CT-infected M. nemestrina and human patients detected Pkn1 and OmpA (~70 kDa and ~35 kDa, resp).
Therefore iTRAQ analyses were performed on sera pooled from 5 animals at each infection stage.
Sera pooled from 3 mice of each study group were subjected to ELISA analysis of cytokine production.
Using these sera, only FCR3-CSA IE were recognized by sera pools of malaria-exposed women in a parity dependent manner (Fig. 6B and C).
For assays that employed immune sera, neutralization titers were calculated based on virus production in wells containing sera pooled from four naive RM as negative controls.
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