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Now, technological advances in sera evaluation, B cell stimulation, microneutralization, and antibody cloning have allowed Burton and colleagues to identify two broadly neutralizing monoclonal antibodies, PG9 and PG16, which provide insights for HIV vaccine design.
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For initial evaluation, sera from each treatment group were pooled, and the IFA method used to determine levels of NiV-specific antibodies.
IS contributed to collection of patient sera and data evaluation.
Twenty-eight days after immunization, sera were collected for evaluation.
The results with the in-house sera panel warranted additional evaluation.
Some subsets of clones which have been tested positive with several patient sera were used for evaluation of protein microarrays.
CLA arrival to pups was confirmed by its quantification in 21-day-old plasma; moreover, immunomodulatory action of CLA in these animals by means of evaluation of sera Ig concentration, and in vitro Ig production and proliferation by isolated splenocytes was also previously observed [ 15].
Automated evaluation reported 546 sera (59.1%) as positive, 140 sera (15.1%) as weakly positive, and 238 sera (25.8%) as negative in regard to the presence of ANAs.
Retrospective evaluation of 1260 sera from patients with ocular symptoms of AR tested for anti-retinal AAbs revealed 819 seropositive samples.
The evaluation of the sera on the whole cell lysate-immunoblot was determined by their responses to PEB1, PEB2, PEB3 and PEB4 antigens [ 23].
Circulating IC in serum and PEG IC-induced IL-10 production correlated in samples from active SLE (r = 0.55, P = 0.003), and we therefore used circulating IC as a surrogate marker for IC-induced IL-10 production in statistical evaluation of all sera with known antibody status.
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