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We sequentially trim one color and re-map, to a minimum read length in our case of 19 colors.
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For each dataset (GEO GSM639446; GEO:GSM639447; SRA:SRR039230), we mapped the full-length reads, and then sequentially trimmed the last color of the reads and repeated the mapping procedure for all previously unmapped reads, to a minimum read length of 19 colors.
In order to improve the accuracy of the consensus sequence prediction, Extender can create replicate seeds for a particular seed by sequentially trimming one base at a time from both ends.
Subsequently, two glucoses are sequentially trimmed from the N-glycan by glucosidase I and II, while two lectin chaperons, calnexin and calreticulin, associate with the monoglycosylated proteins to attain their pre-native conformation (Refs 11, 12).
In some cases, fixed frozen limbs or sterna were trimmed sequentially from both sides to expose the central medullar cavity and processed further for whole-mount fluorescence staining.
Raw reads were trimmed sequentially for adapter contamination, A-tails, and minimum quality score (20) and minimum length (20 bp) as described above.
Read contigs were then sequentially aligned to amplification primers by BLAST (parameters -m 8 -a 4 -F F, minimum score 22) to trim reads of these sequences, then to the reference genome (hg19) using SOAP2 (Li et al., 2009 ) (parameters -M 4 -v 2 -r 1 -p 8).
8. Trim their nails.
Solution: trim the mailbox.
Static stability and trim.
Use trimming scissors to trim rounded areas.
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