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Each resulting slice with automated estimates was then visually examined sequentially, slice by slice, in detail.
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For the retrieval of antigens, detection was performed in 5 μm-thick sections from sequentially sliced samples of paraffin-embedded specimens.
Immunohistochemical analysis was performed in 5-μm-thick sections from sequentially sliced samples of formalin-fixed and paraffin-embedded specimens according to the method described by Byrd [ 24, 26] with slight modification.
Also, we elected to use TMA because they provide a high-throughput technique for the molecular profiling of tissue specimens strengthened by the availability of sequentially sliced samples from the same master block to test the expression of the relevant macromolecules [ 16].
Gel lanes were cut sequentially into slices of approximately 1.5 mm and transferred to a 96-well low binding PCR plate.
Prediction Using Time Series Data We initially sliced the data sequentially into folds (sliced them based on their order in the dataset) and used the cross-validation method in the regression.11 We found that the performance metrics of the generated prediction models deviate considerably.
In the whole-brain task, eye-gaze data was recorded while participants sequentially viewed 60 slices of a patient's brain MRI and in the independent-slice task, both eye-gaze data and performance measures were recorded when participants inspected 32 brain MRIs (16 tumorous; 16 healthy).
Protein bands were sequentially and vertically cut into 10 slices, and each slice was placed in a separate Eppendorf tube.
Typically, this involves using a mouse or keyboard to translate through a stack of parallel slices, sequentially displaying each in turn on a 2D screen for detailed examination.
Once raw data were processed to correct for movement and drifts, in both fluorescence and white light mode, 3D reconstructions were calculated by sequentially stacking 2D slices reconstructed using a filtered back-projection algorithm with a Hann-Filter implemented together with an inverse Radon-transform [20].
The tumor sample was then sliced sequentially into sections 3 5 mm thick.
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