Sentence examples for sequentially over night from inspiring English sources
After removal of any residual annulus fibrosus, the NP tissues were enzymatically digested sequentially over night according to established methods previously reported [ 4, 5, 20].
Seedlings were placed at 25°C, in the dark, over-night.
Hybridization was performed over-night at 60°C with continuous agitation.
Plates were evaluated after an over-night incubation at 37°C.
Blood samples were stored in −70°C after over-night fasting.
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After blocking with 3%H2O22 and Avidin and Biotin (Vector Laboratories , Inc. for 15 min each, slides were sequentially incubated with the Ki-67 antibody (rabbit Ki-67 abcam67, abcam, 1 500 dilution, over night at 4°C) and after washing with PBS with biotinylated anti-rabbit IgG (Jackson ImmunoResearch, West Grove, PA, USA; 1 h at room temperature).
One hundred μL were sequentially diluted until 1 104 and cultured in a single repetition on CLED agar plates over night at 37 °C.
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Scaffolds were kept in distilled water over night.
