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Cross-talk between mediators must be considered and it is possible that multiple proteins will need to be targeted either sequentially or together.
To test this, kan1 and arf4 mutations were introduced sequentially or together into the 35S pro ::FIL GR background and their phenotype assessed following continuous exposure to DEX.
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In particular, the biggest advantage is that tie-up companies can collect market and customers information exceeding the frame of their own type of business, and to use the information for so-called market basket analysis, which is one of the marketing analyses in order to identify purchase patterns (e.g., to identify what items tend to be purchased together, sequentially or by seasons).
Two AFPs P i and P j can be sequentially chained together if P i ≺ s P j or P j ≺ s P i.
The self-assembly consisted of two steps: firstly, nanoparticles sequentially aggregated together by coalescence mainly through (100) and (010) planes to form larger mono-crystalline plates, followed by Ostwald ripening to smooth their surfaces; secondly, the surface-smoothened plates stacked face to face with each other along (001) direction to construct the long chains.
Shown in three phases, "Emergence," "Mutation," and "Cognition," each force comes together here in the form of Kerr, Bjerre, and Clark's meeting of minds: "Each stage of Primordial has been designed and animated by separate artists and sequentially stitched together as one evolving timeline," the video description explains.
Consequently, the respective gene products popped out by the restriction digestion of individual vectors were sequentially ligated together as a single gene and finally into pRSET-A to form chimeric gene SRH as pRSET-A-SRH.
To generate subcellular localization fusion vectors used for experiments in Figure 4, the appropriate cloning vector and an mEmerald fusion vector were digested, either sequentially or doubly, with the appropriate enzymes and ligated together after gel purification.
To generate fusion vectors, the appropriate cloning vector and an EGFP fusion vector were digested, either sequentially or doubly, with the appropriate enzymes and ligated together after gel purification.
When a section is entered while viewing the video sequentially or after a jump, an entry is stored in the database together with the current date and time.
Few if any commentators in the "paradigm wars" suggested that there was anything prohibiting the use of both approaches in the one research program provided that if both were used, they only were used sequentially or in parallel, for they were underwritten by different epistemologies and hence could not be blended together.
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