All the tubes were further plated in 96-well plate by 100 μL in each well sequentially and maintained for 6 h at 37 °C in the incubator.
Aliquots of JP2 (100 μl of a 200 nM JP2 solution) at a flow rate of 50 μl/min were sequentially added, maintaining the same flow rate until there was no further change to the frequency, which was taken to indicate that the there was full coverage of the SLB, i.e. all binding sites were occupied.
Randomization was based on computer-generated codes maintained in sequentially numbered, opaque envelopes.
The positive association seen between having one or more health needs and alternative medicine use in the unadjusted analysis is maintained after sequentially adjusting for the external environment, predisposing factors, enabling factors and barriers to conventional care, and personal health practice controls (models 1-4).
In our case, we can observe that at least two pumps are able to be sequentially overexpressed for maintaining the survival of the bacteria under high level concentrations of chloramphenicol.
Skeletal muscle regeneration is a coordinated process in which several factors are sequentially activated to maintain and preserve muscle structure and function after injury.
Patients were randomised in a 1 1 ratio to the intervention and control arms using permuted blocks of four and six, and allocation concealment was maintained by using sequentially numbered, opaque, sealed envelopes created according to published recommendations [ 11].
To further validate that GCSF induces an increased Tfap2c expression compared to LIF, we examined Tfap2c expression by sequentially giving GCSF to B6-S3Y118F maintainedtained in LIF.
However, providing smaller amounts of ammonium sequentially in the presence of nitrate maintained the pH of a Chlorella vulgaris culture for improved growth on 0.3 gN/L to 5 gDW/L under 5% CO2 gas-phase supplementation.
In a clinical trial of chest radiography in ambulatory children, concealment was maintained by means of sealed sequentially numbered manila envelopes.
The story is told in three parts that do not occur sequentially, but the narrative logic is still maintained, through compositional devises.
