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Eukaryotic transcription involves a multitude of complexes that sequentially assemble on chromatin under the influence of transcription factors and the dynamic state of chromatin.
We used pDSAY and pDSAR to sequentially assemble the seven inserts needed to generate each of the two TALEN transgenes used to obtain mutant mosquitoes in the immunity gene TEP1 (Smidler et al. 2013).
DNA replication initiation is precisely controlled through a core set of licensing factors (Orc1 6, Cdc6, Cdt1, Mcm2 7) that sequentially assemble into pre-replicative complexes (pre-RCs) at ∼30,000 replication origins scattered along the chromosomes, resulting in chromatin being 'licensed' for replication in the subsequent S phase.
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Highly conjugated molecular units are sequentially assembled within the electrode gap by selective design of the molecular end group chemistry.
When NaYF4 Yb,Tm NCs were coated with a TiO2 shell and sequentially assembled with g-C3N4 nanosheets (NSs), a semiconductor heterojunction can be fabricated on the UC particles.
CuInS2 and CdS quantum dots (QDs) are sequentially assembled onto mesoporous TiO2 film to prepare a CuInS2/CdS co-sensitized photoelectrode for QD-sensitized solar cell (QDSC) application.
The sequentially assembled cell-binding NLP with a zwitterionic surface gave a larger transfection yield than the cationic NLP at all concentrations tested.
When model bienzymes, CYP1A2 and CYP3A4 isozymes, were sequentially assembled on Au/CS/RGO, one pair of well-defined redox peaks at -0.531 and -0.474 V (vs. SCE) was observed due to the overlap of the redox peaks of CYP1A2 and CYP3A4, confirming a good electrochemical activity of the CYP bienzyme complex.
First, O-units are sequentially assembled onto lipid carriers in the cytoplasm.
This has revealed that Drosophila core kinetochore complexes are sequentially assembled onto the centromere in a hierarchical fashion similar to other eukaryotes.
Enzymes called glycosyltransferases are responsible for sequentially assembling a complex array of sugar molecules on dystroglycan.
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CEO of Professional Science Editing for Scientists @ prosciediting.com