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An over-expression E. coli strain was constructed by transforming E. coli ER3081 (NEB) with pSYX20-hpyAVM and pAII17-hpyAVR by sequential transformation (See Materials and Methods).
Indeed, the bulk for the oxygen requirement centers on the sequential transformation of lanosterol to cholesterol, involving several redox reactions.
AES consists of 40 sequential transformation steps composed of simple and basic operations such as table lookups, shifts, and XORs.
This idea, also known as the retrograde hypothesis, has intuitive appeal and states that if the contemporary biosynthesis of compound "A" requires the sequential transformation of precursors "D," "C," and "B" through the corresponding enzymes, the final product "A" of a given metabolic route was the first compound used by the primordial heterotrophs (Fig. 8).
The past several decades have seen dramatic progress in understanding of pancreatic cancer pathogenesis, including identification of precursor lesions, sequential transformation from normal pancreatic tissue to invasive pancreatic cancer and corresponding signature genetic events, and the biological impact of these events on malignant behavior.
Our model can be readily extended to include the growth of the domain and study precisely this sequential transformation.
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Nevertheless, in order to saturate screening, it is advisable to perform sequential transformations which mostly results in a few million transformants.
Several families of compounds are synthesized by sequential transformations.
The α- and δ-relaxation times were found to be sensitive to the sequential transformations between mesophases.
Two other methods using computationally-predicted metabolites, MyCompoundID [25] and Ridder et al.'s green tea metabolites [27], begin with much smaller metabolite starting sets than KEGG COMPOUND but utilize broader reaction rules and permit more sequential transformations.
The sequential transformations are (1) alkane bromination to alkyl bromide(s) and HBr, (2) reaction of this mixture with solid metal oxide (MO) to neutralize the HBr and to generate oxygenated products or olefins plus solid metal bromide, and (3) oxidation of the spent solid with O2 to regenerate the metal oxide and Br2 for reuse.
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seamless transformation
evolutionary transformation
successive transformation
orderly transformation
steady transformation
consequent transformation
incremental transformation
progressive transformation
frequent transformation
gradual transformation
sequential situation
sequential decline
sequential organ
sequential growth
sequential liability
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