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Seven sequential strain images, each 3 weeks apart, were collected in total.
Specific emm sequence types of GCS/GGS did not appear to cycle through the communities (sequential strain replacement) in a manner suggesting acquisition of type-specific immunity.
The community pattern of sequential strain replacement seen with GAS (24, 29 ) was absent, which suggests that acquisition of M protein type specific immunity against GCS/GGS may not play a role in these communities.
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The treatments included five sequential strains, separated by pauses, in a temperature range of 950 to 1150 °С.
This often includes sequential straining and annealing at elevated temperatures followed by subsequent aging heat treatments at lower temperatures.
The LPS of P. aeruginosa sequential strains AA2, isolated at the onset of chronic colonization, and AA43 (mucoid) and AA44, isolated before patient's death (Figure S1), was extracted by conventional methods (see Supporting Information File S1) and showed the typical ladder like pattern by SDS-PAGE electrophoresis (data not shown).
Taken together our results, obtained by comparing sequential strains isolated from one CF patient, showed that the synergy of the action of the two modified P. aeruginosa PAMPs, PGN and lipid A, could drastically impair the CF host immune detection system thus preventing the eradication of the infection with this pathogen.
Sequential strains obtained in the study period from the same patient showed identical macrorestriction profiles.
In sequential epidemics, strains within these genotypes appeared to be of two types: those continuing from the preceding epidemics and those newly introduced.
6 7 31 32 36 41 44 50 Previous data suggested a high eradication rate with sequential therapy in strains resistant to clarithromycin.
The prevalent view of intrinsic mechanisms for cyclical epidemics in the situation of strong cross-immunity is the enhancement in sequential infections between strains [ 38, 59, 62, 63, 75].
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