Here, we describe a simple and inexpensive method, requiring minimum equipment and accessories, to obtain lymphatic (HL-LECs) and blood (HL-BECs) endothelial cells from healthy human lung using a two-step purification tool based on sequential sorting with monoclonal antibodies to CD31- and Pdn-coated paramagnetic beads.
Wüthrich's solution, called sequential assignment, sorts out the tangle by methodically matching up each NMR signal with the corresponding hydrogen nucleus in the protein being analyzed.
Phyfe's cabinetmaking workshop, which at one time employed more than a hundred people, used new, simplified, sequential techniques, a sort of proto-assembly line, allowing him to employ lower-paid, semiskilled workers, many of whom probably lived in nearby boarding houses.
EBOECs and GBOECs were successfully produced by retroviral gene transfer by sequential transduction and sorting by FACS.
ICAM-3-deficient Mutu Burkitt's lymphoma (BL) cells or Jurkat T cells (human leukocyte cell lines that normally express ICAM-3) were isolated following sequential flow cytometric sorting for very low/negative expression levels of surface ICAM-3.
Autarchoglossans developed a sort of sequential polygyny mating system in which a single male guards a number of females as they move about.
Sequential images show a sort of movie of the loops as they emerge from between sunspot regions.
Distribution of organic matter is not random, but is dominated by hydrodynamic sorting and sequential fall-out of particles as turbidity currents passed across the basin.
Mastering a notation's visuospatial structure allows us to transform symbolic, sequential reasoning of the sort found in mathematics or logic into a series of simpler perceptual tasks (Hutchins, 1995; Rumelhart, Smolensky, McClelland, & Hinton, 1986).
These can be found in any order, as the game world is effectively an open one (save for its final sequence), but assuming you follow each of the game's character- and chapter-specific orbs of light down the lanes and up the public footpaths, they generally make a sort of sequential sense.
ICAM-3-deficient Mutu I cells were generated by sequential fluorescence-activated cell sorting of a WT Mutu I population.
