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For static state and output feedback of sequential hybrid systems, the paper proposes a new hybrid sequential sliding-mode controller.
Sequential slides were stained for desmin, a marker for regenerating fibers, and this staining corresponded to Tβ4 staining.
Using an immunoperoxidase method, sequential slides were also stained with a monoclonal antibody directed against the nucleoprotein of IAV [50].
Representative sequential slides were stained with Masson's trichrome stain and H&E for determination of infarct size as previously described [35].
Sequential slides were stained using an immunoperoxidase method with a monoclonal antibody (Clone HB65 IgG2American Type Culture Collectionion)) directed against the nucleoprotein of influenza A virus. a Goat-anti-mouse IgG2a HRP (Southern Biotech, Birmingham, Alabama, USA) was used as secondary antibody.
Sequential slides from those used for TRAF6 staining were then stained for the aforementioned CD markers.
Particular care was taken to mark the sequential slides of each case identically, so as to score corresponding fields for the three different antibodies.
(21) Aggregates of bone marrow plasma cells that were CD138 positive (the protocol for immunostaining is described below) were evaluated for LAT1 and CD98 expression in sequential slides.
One was stained with hematoxylin and eosin and two sequential slides were used for the immunohistochemical detection of APRIL and BAFF.
In these 15 representing clinical specimen, the expression pattern of ADAM17 was similar by the monoclonal antibody and the polyclonal antibody when compared in sequential slides (Supplementary Figure 5a f).
Due to the co localization of the CD3 and PD-1 molecules in the T lymphocyte membranes, double staining of PD-1 or B7-H1 and CD3 were assumed based on single-staining of sequential slides.
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