Sentence examples for sequential purification from inspiring English sources

We prepared E-PS samples with varying dodecyl sulfate surfactant concentration made via sequential purification.

For purification, carbon soot was collected in a thimble and then placed in a soxhlet extractor for sequential purification with petroleum ether, acetone, ethyl alcohol and finally with water.

Using a sequential purification protocol involving a Ni2+ affinity column followed by a UDP-hexanolamine affinity column, we have obtained a pure and active protein from the soluble fraction which catalyzes the transfer of galactose (Gal) from UDP-Gal to N-acetyllactosamine (LacNAc) with a specific activity of 0.69 pmol/min/ng.

Sera were collected from long-term non-progressor patients to obtain CD4bs-directed IgG, through sequential purification steps.

Sequential purification first on Protein A followed by Protein L enabled isolation of antibodies that contain both heavy and light chain, thus eliminating any unpaired heavy or light chain along with degraded fragments and contaminating bacterial proteins (Figure 4B).

A three-step affinity purification protocol was developed that involved sequential purification of His6 TPL-2 and ABIN-2 StrepII ABIN-2 StrepIInity tags.

Cell culture supernatants were screened by enzyme-linked immunosorbent assay and Western blot for selection of high expressing clones followed by sequential protein purification with anion exchange and hydroxyapatite columns by fast performance liquid chromatography (FPLC) [5].

The TAP-tagged protein and any associated proteins are then isolated from the host organism in two sequential affinity purification steps.

For sequential affinity purification of LRP6 prior to Western analysis, HEK293 cells were transfected with 0.33 µg/ml each of LRP6-flag and LRP6-HA expression constructs using FuGENE 6 reagent (Roche), treated 1 day later with 1 µg/ml antibody for 1 h on ice, washed with PBS, and lysed in SJC buffer containing 1% n-dodecyl-β-D-maltoside (DDM).

Proteins associated with CSB TAP were isolated through two sequential affinity purification steps, as described in Materials and Methods.

From the highest dilution plates, representative bacterial colonies from the predominant colony morphological types were subject to several rounds of sequential colony purification.