Sentence examples for sequential overlap from inspiring English sources

Herein we present a simple, universal, efficient gene synthesis method based on sequential overlap extension polymerase chain reactions (OE-PCRs).

For sequences longer than 100 residues, two sequences in an alignment were considered identical if the sequential overlap and the sequence identity within the overlap were both 99% or greater.

The PCR products were fused together by sequential overlap extension, digested with XhoI and NotI, and ligated into pKS149 cut with SalI and NotI to give pKS152.

PCR products were column purified with NucleoSpin® Extract II kit (genXpress) and fused in a sequential overlap extension resulting in the ARE2 deletion cassette (3885 bp).

The two PCR products were fused together by sequential overlap extension, digested with MfeI and HindIII (pyc, aceE, and pgi fragments) or with HindIII and EcoRI (ppc and ilvE fragments), and ligated into pK19mobsacB cut with EcoRI and HindIII.

In case of sequential overlaps between different gene models, the gene models which are of longer length and with RNA or protein evidence for a given region of scaffold were preferred over the ones without any evidence.

Particularly promising for these areas is a material system synthesized by the sequential-overlap method, which produces a networked, transversely isotropic open cell porous material.

In sol-gel processing the pore structure of the dry gel (xerogel) or film is a consequence or the sequential (or overlapping) gelation, aging, and drying stages.

Seven (7) of the 78 sequences had at least 3 sequential nonamers overlapping by 8 amino acids.

Twenty three 15-mer sequential peptides overlapping by nine amino acids and spanning the genomic sequence of the M. tuberculosis (H37Rv) HBHA-protein starting from 37th amino acid were synthesized (Proimmune, Oxford, UK; Alta Bioscience, Birmingham, UK).

In addition, for many individuals the hair samples from sequential visits overlapped with respect to exposure period.