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CRFs have the advantage of modeling the relationships between sequential labels and have been proven efficient in prosodic prediction[2, 11 13].
Tandemly-arrayed genes were given sequential labels, for example, in T. brucei where AAT5 is present with four arrayed copies, these are labelled AAT5.1Tb to AAT5.4Tb.
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It also holds the potential to perform other sequential labelling protocols based on bleaching or antibody removal6,24,25.
Recent DNA-PAINT modalities can reach more than the typical 2 3 channels by using antibodies coupled to orthogonal DNA strands and sequential labelling combined with imaging sequences of PAINT imagers3,17.
CRFs are undirected graphical models that are trained to maximize a conditional probability of random variables, and have been successfully used in numerous sequential labelling tasks, such as named entity recognition and Chinese word segmentation.
Tumor and spleen MDSCs were isolated by sequential labeling and column isolation with anti-Gr1-PE and anti-PE multisort beads, then CD11b beads (all from Miltenyi).
For multicolour labelling of markers, sequential labelling of antibody pairs was used.
The basic task is addressed as supervised sequential labeling using this representation.
One fraction was stained using a multistep sequential labeling protocol for FCM.
Likewise, the IdU-CldU sequential labeling study also indicated delayed DNA replication at heterochromatic domains in Nde1−/−Trp53−/− neural progenitors.
(C ) Validation of equivalent labeling using CoA-dye or CoA-biotin by sequential labeling of F-purified, RRL-expressed MCP-TERT with SFP synthase.
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