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We demonstrate, through a series of examples, optimal topology achieved through sequential insertion of a heterogeneity of fixed shape and optimization of its configuration (location, orientation and scale).
As previously described [21], after surgical preparation and stabilization, 15 mL of a 108 colony-forming unit (cfu /mL suspension of P. aeruginosa ATCC 27853 was inoculated through sequential insertion of a bronchoscope into the main right upper, medium and lower bronchi and the main left upper and lower bronchi.
In contrast, the sequential insertion of He atoms in Σ73b GB continuously emits self-interstitial atoms (SIAs), but these SIAs rearrange at the core of the inherent GB dislocation, instead of forming a dislocation loop, which leads the GB dislocation to propagate along the [¯1¯112] direction.
Persistent strictures were treated by repeated dilation and sequential insertion of multiple stents.
Persistent strictures will be treated by repeated endoscopic dilations and sequential insertion of new stents for a maximal period of 1 year, after which stenting must be stopped.
Subsequently, multipoint linkage analysis was performed to determine the most significant position of the SNPs in each linkage group by sequential insertion of SNPs using the ALL option.
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Typically, these changes involve sequential insertions at a specific site such as tRNA gene as in the case of the fGI1, metal-resistant/hydrogenase island that has been already described (Gonzaga et al. 2012), or specific mobile elements such as an integron (fGI2, see later) or a mobilizable GI (MGI) fGI9.
Initial values for similarity function F for global alignment are specified as: 13 14 15 We chose values ν do to prohibit the optimal path in local and global alignments from moving round the nucleotide having minimal weight in matrix w i, j) because of two sequential insertions.
Their results suggest that the membrane protein insertion/folding process is more complicated than commonly depicted in the sequential-insertion scheme.
Because the positive-inside rule was robustly verified in the Stockholm experiments, a logical conclusion is that the sequential-insertion scheme does not describe accurately how EmrE, and perhaps other membrane proteins, fold inside cells.
Given the positive-inside rule and the sequential-insertion scheme, one would expect positive charges in the C-terminal region of a membrane protein to have a smaller influence on topology than charges in the N-terminal region.
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