Gradual acceptance of the notion that sequential sleep hours are not essential for high-level job performance has led to increased workplace tolerance for napping and other alternate daily schedules.
To test the feasibility of using BMP-12 as an inducer of BM-MSC tenocyte differentiation, we first compared the effects of a single 12 hour treatment and 2 sequential 12 hour treatments with 10 ng/ml BMP-12 on the expression of Scx and Tnmd in rat BM-MSC monolayer cultures.
Duplicate reactions of Mowry shale were carried out in four sequential one-hour reactions at 100°C and a fifth reaction at 100°C for 24 hours (Table 3).
The ELISA was developed the following day by sequential one hour incubations with biotinylated 6H9 reporter antibody (Elan, Aβ 17-28 specific, 0.25 μg/mL) for one hour at room temperature, and Streptavidin-Alkaline Phosphatase Conjugate (Roche Molecular Biochemicals, Catalog No. 1089 161, Indianapolis, Indiana, USA), 1 1,000 in specimen diluent, one hour room temperature.
Comparison of PaCO2 evolution during two sequential 2-hour periods of ventilation (standard ventilation and Intellivent-ASV® version VUP02.11c in the "brain injury setting"), applied in random-order.
Lowe is joined by Ebro Darden and Julie Adenuga as full-time DJs, and the service also promises a rotating collection of guest DJs and other special content – they aren't each just settling in for three sequential 8-hour shifts, in other words.
During three sequential two-hour meetings spread over the course of a week, we conducted a moderated focus group using a brainstorming and consensus building technique [23] to identify all factors (at any granularity of detail) that impact network risk perception.
The G1 and G2 phases of the cell cycle lead and followed the S phase, with some overlap, but on the whole the population displayed remarkable and reproducible synchrony, both between the replicate cultures and over sequential 24 hour periods.
The preparation was subsequently dialyzed for two sequential 16-hour intervals in 100 mM Tris, 400 mM arginine, 2 mM β-mercaptoethanol, 2 mM EDTA, pH 8.0 and 100 mM Tris, 200 mM arginine, 2 mM β-mercaptoethanol, 2 mM EDTA, pH 8.0.
Organ dysfunction was categorised with 24-hour Sequential Organ Failure Assessment (SOFA) scores.
