Sentence examples for sequential generations of from inspiring English sources

After three rounds of error-prone PCR and three sequential generations of DNA shuffling were performed on the glyA gene and recombinants were screened using a genetic complement coupled assay, a mutant 3E7 with approximately 8-fold increased enzyme activity and 41-fold increased enzyme productivity compared with its wild-type parent was obtained.

Under double selective pressures of activity and thermostability, through two rounds of error-prone PCR and three sequential generations of DNA shuffling, coupled with screening, a mutant pepEM3074 with approximately 47-fold increased enzyme activity compared with its wild-type parent was obtained.

These were in the same range as the parental cells (81.3∶ 18.7), demonstrating similar CD44 expression hierarchy of sequential generations of xenografts (Fig. 3B).

In this case, sequential generations of inoculation and re-isolation in a serial passage experiment should lead to selection for strains with increased infection efficiency, without regard to how much necrosis was produced.

In addition to WbnH, WbnJ, WbnK and WbnI also contribute to the sequential generation of the repeating unit pentasaccharide-PP-Und (Woodward et al., 2010).

A design method presented in a previous paper for the sequential generation of observation sites used for the inversion of a prediction model is extended to cope with practical issues such as delayed observations and design of batches of imposed size.

The intrinsically controlled sequential generation of neurons and glial cells seems to be also preserved in ES cell-derived NPs.

This time course closely recapitulates the sequential generation of NSCs, NPCs and neurons observed in vivo (Fig. 1A).

In conclusion, our experimental data suggest that extravasated plasmin ogen) mediates firm adherence and (subsequent) transmigration of neutrophils to the reperfused tissue indirectly through activation of perivascular mast cells and a sequential generation of leukotrienes and PAF.

Important points that should be taken into consideration in the sequential generation of ligand molecules concerning the placement of fragments on the surface are discussed by Pegg et al. [29].

This is compatible with former fate-mapping experiments, which suggest a sequential generation of cotyledons [ 45].