Sentence examples for sequential buffer from inspiring English sources

In vitro studies at 37 ± 0.5 °C temperature under sequential buffer conditions showed controlled drug release with respect to the variation in pH values while following Eudragit VR L100 coated gelatin capsules; however, dialysis bag technique do not show such pH controlled delivery.

Guanidine hydrochloride supernatant was dialyzed against urea buffer for 4 h and pooled with urea supernatant followed by a slow sequential buffer exchange to refolding buffer (50 mM Tris HCl, pH 7.5, 50 μM ZnO Ac 2, 5 mM DTT).

The presence of TTR or Aβ in the initial flow through (FT), sequential buffer washes (W), and sequential guanidine hydrochloride elution steps (E1 E2 and E3 E4) (x-axes) was detected by the integrated peak density of the dot blot analysis (y-axes).

After injecting sequential buffers, DNA could be rapidly isolated and released from the back side of the NMM, providing fast and highly efficient DNA isolation for diagnostics based on DNA sequence.

Protein pellets were washed three times with 100% methanol, air-dried for 5 min, and then re-dissolved in Sequential Extraction Buffer 3 (Bio-Rad, Hercules, CA, USA) for gel electrophoresis or sterile Milli-Q water for other assays.

Approximately 200 μg of protein (re-suspended in 180 μL Sequential Extraction Buffer 3) was applied to an immobilized pH gradient (IPG) strip (110 mm, pH 3-10, nonlinear, Bio-Rad) and actively rehydrated.

To rule out extraction artifacts, we extracted a different set of human gray matter tissue (AD N = 10, age 77.9±7.7 years, MMSE 4.9±5.4/Ctrl N = 10, age 77.0±8.2 years, MMSE 29.3±1.0) with sequential extraction buffers yielding a cytosolic fraction (RAB buffer) and a membrane bound fraction (RIPA buffer).

The other sample was homogenized and subjected to sequential extraction buffers of increasing protein extraction strength.

Distance-related attack rates and relative risks for increasingly larger sequential ring buffers from each of the 7 large farms (A-G) are shown in Table 1.

Western blots of total brain protein (RIPA) and sequential extraction buffers (high salt, high salt/Triton X-100, SDS and formic acid) of increasing protein extraction strength were performed to examine solubility state.

To isolate fractions enriched in various cell wall components, AIS were subjected to sequential extraction buffers (at final concentration of 30 mg/ml) in constant mixing for 24 hours at room temperature.