Sentence examples for sequential backbone from inspiring English sources
For very large proteins in the highest magnetic fields, the large chemical shift anisotropy (CSA) of carbonyl carbon deteriorates coherence transfer efficiency in experiments designed for unambiguous sequential backbone assignment.
Because we suspected conformational disorder was hindering crystal growth, we turned to solution NMR and obtained the sequential backbone assignments of wt, Y75A, and H83A apo-HasAp.
A suite of five experiments (see Experimental Procedures) allowed sequential backbone assignments for 93% of non-proline residues in wt apo-HasAp (Table S1 of the Supporting Information).
Hence, a suite of triple-resonance NMR experiments was conducted on [U-H, N, C]-Fe3+-HO2O for sequential backbone assignments.
Sequential backbone resonance assignments were achieved by using standard 15N-1H-HSQC, HNCBCA CO NHO)NHBHA CO NH)NOESYOESY (nuclear Overhauser enhancement spectroscopy -HSQC, and TOspectroscopy -HSQCted spectroscopy)-HSQC experiments.
Sequential backbone assignments of the U-N,C-labeled DC-SIGN CRD (500 μM) were made at 37 °C using three-dimensional intra- and inter-residue coherence transfer experiments, HNCA, HNCB (HNCACB with tauCC = 7 ms), and HN CO CACB.
Backbone sequential and side-chain assignments were obtained using standard 3D NMR experiments [50].
Comparison of structure-based and triple resonance sequential assignment procedures : backbone assignment of proteins is usually performed using triple resonance experiments.
In this work, we functionalized a polyester backbone via sequential Huisgen 1,3-dipolar cycloaddition and 3-component Passerini reactions.
They are not explicable using most other tools, such as the 〈 ψ3〉 KD chain profiles, BLAST sequential similarity, or conventional backbone Euclidean geometrical structural superpositions.
By docking capsaicin to the closed state, or to open states with or without backbone movements, the sequential events underlying capsaicin binding and activating TRPV1 started to emerge.
