Sentence examples for sequencing verification from inspiring English sources
Following PCR and sequencing verification, the expression and subcellular localization of the proteins by these viral vectors were analyzed.
As the phenotype of lf1132 was similar to the phenotype of se1 mutants, we investigated whether lf1132 harbored a mutation of the Hd1 locus through sequencing verification of lf1132 and Zhonghua 11.
Reaction treatments, RT-PCR protocols, product cloning and sequencing verification were performed as described [ 5, 6].
For Sanger sequencing verification of genotypes called by NGS, oligo-nucleotide primers were synthesized by Integrated DNA Technologies.
Another technical improvement is the simplified construction of shRNA expression plasmids that allow omitting colony selection and sequencing verification.
After the DNA sequencing verification, the insert was subcloned into pGL3-basic reporter vector within XhoI/ HindIII sites.
Sequence verification was performed using a CEQ capillary sequencer (Beckman Coulter, Fullerton, CA).
Sequence verification of PCR-generated fragments was performed by sequencing.
Following sequence verification, the fragments were cloned into the pCAMBIA1301 binary vector (CAMBIA; www.cambia.org.au).au
High Resolution Melting (HRM), amplicon size quantitation and sequence verification is used to screen for non-target amplification (false positives).
After sequence verification and LguI/Eco81I double digestion, genes were ligated into LguI-linearized pQE-30-LE to give plasmid pQE-30-LE::bgl1 (Eurofins, Ebersberg, Germany).
