Sentence examples for sequencing vector from inspiring English sources

In addition all bands were cloned by TopoTA cloning according to the manufacturer's recommendations into the pCR®4-Topo sequencing vector and sequenced with T3 and T7 primers (all: Invitrogen, Karlsruhe, Germany).

Genomic DNA of F. nucleatum ATCC 10953 was sheared to 2 6 kb in size using a nebulizer (CIS-US, Inc., Bedford, Mass ., purified from an agarose gel, cloned into a pUC18 derivative sequencing vector, and sequenced as previous described [94].

Amplicons were cloned into a sequencing vector, and full-length ORF sequences were obtained.

The PCR product was gel eluted and ligated to sequencing vector yT&A (Sigma).

All sequences were previously trimmed to remove sequencing vector, cloning vector and Escherichia coli contaminant sequences.

Sequencing was performed on PCR products that were directly hydosheared and cloned into a sequencing vector or were first cloned into the pCR®4-TOPO vector from Invitrogen before hydoshearing and subcloning into the sequencing vector.

PCR fragments were cloned into sequencing vectors (pJet & pGemT-Easy) and sequenced.

Hits to common plasmids and sequencing vectors were removed (using data from NCBI Univec).

Sequencing vectors from these cells identified nine IFP that were fragmented distal from the termini (>25 residues).

This chapter covers selection of short hairpin sequences, vector design, production of lentiviral supernatants, transduction of dissociated primary hippocampal neurons, and testing the effectiveness of shRNA-mediated silencing.

After removing low quality sequences, vector trimming, clustering and contig assembly, there remained 1437 clusters (285 contigs and 1152 singletons).