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It supports the production engineer throughout the entire planning process, departing from part analysis via task sequencing up to the generation of detailed work instructions.
Microbial diversity was assessed by sequencing up to 24 clones per subject.
We next evaluated how the FRM performs with independent datasets by firstly using another publicly available CD4+ T cell methylation dataset generated using the"Illumina GoldenGate Array for Methylation", designed for sequencing up to 1,536 loci [48].
We analyzed the methylation levels by sequencing up to 20 individual bacterial clones containing bisulfite converted DNA.
The cost of PCR amplifying and Sanger sequencing up to 100 candidate regions is small compared to the cost of resequencing itself.
Genetic variability within single individuals was determined by sequencing up to five clones per individual and analysing PCR products obtained from several individuals per morphospecies where possible.
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We sequenced up to 24 rRNA clones per positive specimen and assigned taxonomic designations to approximately the species level.
Using non-paired end sequencing chemistry, strains were sequenced up to a depth of 15x and assembled de novo by the 454 Newbler assembler.
Sequencing clean up was done using ZR DNA Sequencing Clean up Kit according to the manufacturer (Zymo, USA).
Almost 20 vertebrate genomes have been fully sequenced up to date.
Sequences with unique or rare haplotype were amplified and sequenced up to three more times in both directions.
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