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Our data should help researchers who are planning exome sequencing to select appropriate exome capture technology for their particular application.
Taking into account the ability of direct sequencing to select one strain only, it seems possible that, in such cases, the previously adapted method could also find only a single gB2 strain of HCMV [ 24].
PBS values can be used with gene length, ease of amplification and sequencing to select a suite of genes for phylogenetic inference, especially given that different suites of genes may provide more information as a function of the tempo of evolution.
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In the other approach, computational modeling is used to explore an astronomically large number of protein sequences to select a small number of sequences for experimental testing.
Our experiments provide direct evidence of the ability of indirectly presented allochimeric sequences to select the TCR repertoire and thus provide a powerful tool for manipulating the immune response through the sequences of the indirectly presented antigen.
Second-strand cDNA synthesis was done with a single round of primer extension using a 5' trans-spliced leader primer conjugated to Clontech's primer IIA sequence to select for full-length dinoflagellate transcripts.
One approach was to use overlapping BAC genomic sequences to select polymorphisms that varied by more than two repeats [ 15].
This was applied to the 4,067 unique sequences to select primer pairs that would produce single products.
A second alignment was performed with flanking sequence to select all sites with 5' LTR sequence, complete IAP structure, and 3' LTR sequence.
Projection projects all l-mers from the input sequences onto many buckets by hashing and then derives the consensus sequences to select some valid buckets.
Bisulphite-converted DNA from cancer cell lines and normal tissues was first sequenced to select a representative region for MS-MLPA probe design (see Methods).
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