The recent boom of sequenced cancer genomes illustrates the potential of next-generation sequencing to provide unprecedented insights into the mutational processes, cellular repair pathways and gene networks associated with cancer.
Recent studies have used single cell expression and high-throughput immunoglobulin sequencing to provide a detailed picture of antibody responses.
Yin's team will harness all of these features to develop a method that is able to fingerprint single proteins in cells, complementing next-generation DNA and RNA sequencing to provide a more complete picture of cell identities and functions.
Yin's team will harness the sum of these features to develop a method that is able to fingerprint single proteins in cells, complementing next-generation DNA and RNA sequencing to provide a more complete picture of cells' identities and functions.
The described approach illustrates the capacity for amplicon sequencing to provide ecologically relevant information that can be used to strengthen an understanding of how sedimentary communities respond to a range of environmental stressors.
In addition to identifying research gaps, the discussion of these manuscripts underscores the important practical issues of implementing rapid turnaround time between virus isolation and sequencing to provide real-time surveillance.
While originally limited to microarray technology, metatranscriptomcs studies have made use of direct high-throughput cDNA sequencing to provide whole-genome expression and quantification of a microbial community, as first employed by Leininger et al. (2006) in their analysis of ammonia oxidation in soils.
We therefore sought to explore the potential of deep sequencing to provide additional, clinically relevant genotypic information that could be used to improve treatment decisions in heavily pre-treated HIV-1-infected subjects, relative to population sequencing.
All nine adults underwent paired-end sequencing to provide longer templates to improve annotation (Table S1).
Five BACs were selected for sequencing to provide external validation of the shotgun genome assembly.
Therefore, we performed sodium bisulfite sequencing to provide the methylation status of all CpG within the amplified target fragments.
