Sentence examples for sequencing to obtain from inspiring English sources

The products were then used for next-generation Roche GS FLX Titanium sequencing to obtain metatranscriptome data.

Each single colony was analyzed by the mutation-sensitive T7 endonuclease I assay, fluorescent PCR, and dideoxy sequencing to obtain three independent clonal populations of cells that contained biallelic modifications.

We used ultra-deep sequencing to obtain tens of thousands of HIV-1 sequences from regions targeted by CD8+ T lymphocytes from longitudinal samples from three acutely infected subjects, and modeled viral evolution during the critical first weeks of infection.

These coding regions were then selectively assayed by sequencing to obtain haplogroups and their derivatives (Fig 5).

To address these issues, we characterized MGMT promoter methylation patterns using bisulfite sequencing to obtain quantitative methylation results for all 97 CpG sites in 70 newly diagnosed GBM patients.

We used directed PCR sequencing to obtain evidence of scaffold linkages.

Then, single-stranded cDNA is directly amplified with oligonucleotides which contain Illumina adaptors and indexes sequences to obtain a ready-to-sequence library.

Primers were designed from the published pCS20 sequence to obtain sequences of the pCS20 region of various E. ruminantium isolates.

Standard acquisition sequences to obtain one-dimensional spectra suffer from substantial overlap of spectral contributions from many metabolites.

The basic principle of this procedure is characterized by tailoring of short wave sequences to obtain a certain maximum response.

Mark Achtman, an expert on plague who works at University College Cork in Ireland, said that the new study was "technologically interesting" but that a great deal more of the microbe's DNA needed to be sequenced to obtain scientifically important results.