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The efficiency of the mcrA enrichment was determined by cloning and sequencing the second capture product.
Sequencing the second allele did not identify any coding abnormalities, indicating that the boys were not compound heterozygotes for coding variants.
WGS will be carried out in one individual from each of the 1,500 relative pairs (genome sequencing the second individual would provide little additional information, since the relatives are so closely related, but the second individual will be valuable for testing for the presence of proposed pathogenic variants).
In order to ensure the specific identity of the specimen prior to sequencing, the second internal transcribed spacer (ITS-2) of nuclear ribosomal DNA was amplified from the genomic DNA sample by the PCR and sequenced directly.
To simplify identification of different species by DNA barcodes, a target DNA sequence marker has to meet two basic requirements: the first is a high success rate during PCR amplification and DNA sequencing, the second is sufficient DNA sequence polymorphism to permit different species to be distinguished and evolutionary distances between them to be calculated [ 1].
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He pioneered methods for sequencing the first bacterium, Haemophilus influenzae, and raced the government to a draw in sequencing a draft version of the human genome in June 2000.
The cost of sequencing the first genomes — determining the exact order of the base pairs of a segment of DNA — were astronomical.
DNA sequencing costs have fallen from about $3 billion, the investment in sequencing the first human genome in 2001, to about $1,000 today.
By sequencing the first ophioglossoid fern and a second horsetail (E.
After sequencing, the first step involves mapping those short reads to a genome or transcriptome.
Dr. Rothberg sequenced the first individual human genome (The Watson Genome, Nature), and initiated the Neanderthal Genome Project with Svante Paabo.
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