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The MFSTAAD chip is an alternative tool to next-generation sequencing that allows parallel analysis of several genes on a single platform.
Recent advances in sequencing technology have led to increased sensitivity in sequencing analysis (deep sequencing) that allows even lowly abundant small RNAs to be detected [ 15].
We present a tool based on whole genome sequencing that allows detection and quantification of coexisting genotypes mediated by genomic inversions in bacterial cultures.
If one is interested in the functionality of a particular RNA sequence within the mutant spectrum of the quasispecies, it can be picked-up from the population following a protocol based on molecular cloning and sequencing, that allows the sequence analysis of both majority and minority genomes of the quasispecies, as recently exemplified with human immunodeficiency virus [ 50].
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The Berkeley research has been enabled by new technologies, such as inexpensive gene sequencing, that allow scientists to search for a multitude of rare variants unlikely to be detected with other genetic tools.
After all, it was the introduction of high-speed machines for DNA sequencing that allowed quick-moving and well-financed companies such as Incyte to amass private empires of genetic data.
The work became feasible only in the past few years because of enormous advances in DNA sequencing that allow researchers to scan all the DNA in a cell instead of looking at its 21,000 genes one at a time.
Barley researchers are, however, benefitting from advancements in genetic sequencing that allow them to evaluate a line's potential without going through the lengthy process of growing it.
Emerging methods of massive sequencing that allow for rapid re-sequencing of entire genomes at comparably low cost are changing the way biological questions are addressed in many domains.
These carry both an antisense sequence that allows specific binding to exon 7 and a splicing enhancer sequence that will improve the recognition of the targeted exon.
We have designed a P. pastoris expression vector, pBGP1, incorporating an autonomous replication sequence that allows the plasmid to exist as an episomal element.
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