Sentence examples for sequencing templates from inspiring English sources

A pair of primers, ORF100-INDE were used to amplify the sequencing templates.

Fragments (200 ± 25 bp) were then separated by agarose gel electrophoresis and selected for PCR amplification as sequencing templates.

The technique allows amplification of nucleic acid sequences both for the purposes of disease and pathogen detection and also for the preparation of hybridization probes and sequencing templates.

We have evaluated the performance of new isothermal amplification (IA) reagents for clonal amplification of sequencing templates and Ion PGM TM) Hi-Q TM) sequencing reagents in tHi-Q TMext of HLA sequencing.

Amplification products were diluted 1∶5 in water and used directly as sequencing templates.

Preparation of sequencing templates was carried out following standard protocols, employing FIREPol polymerase (Solis BioDyne).

This protocol was used to obtain sequencing template from reoviruses of three different vertebrate classes.

E. coli cultures were amplified by rolling circle amplification (RCA) using the Templiphi™ DNA Sequencing Template Amplification Kit (GE Healthcare, Piscataway, NJ).

However, when using PCR products obtained by short-range PCR amplification as Sanger sequencing template, electropherograms showed clearly heterozygous peaks for these same SNPs.

When the Excess Primer Strand is used as the sequencing template, there is a very low level of background signal in the chromatogram.

Plasmid DNA preparation was performed with the TempliPhi DNA sequencing template amplification kit (GE Healthcare -Bio-Sciences) or Splasmidepreparationreparation kits (Agencourt Bioscience).